美国Cyto Pulse Sciences, Inc.  www.cytopulse.com
美国Cyto Pulse Sciences, Inc.成立于1995年.是一家专业从事电穿孔、电融合仪器研发和生产的公司，多年来致力于研究用于医疗和商业用途的先进脉冲电场技术系统，并将其成果应用于科学研究。拥有独一无二的 PulseAgile 协议方波、细胞融合和电穿孔专用培养基、控制软件等多项技术。Cyto Pulse 能够提供参数(强度、脉宽、方向、重复频率等)更加快速、灵活变化的脉冲电场，从而实现高效的转化和融合效率。

该公司与全球几十家科研机构建立了长期合作，其产品接受了广泛的测试和改进，广泛的应用于动植物细胞及微生物的转化、杂交瘤、细胞融合等相关研究。
2010年法国Cellectis 收购美国Cyto Pulse Sciences 

Cyto Pulse Sciences, Inc. was founded in December 1995 and is incorporated as a regular C corporation in Maryland. The company is composed of a multidisciplinary staff of engineers and scientists. Cyto Pulse develops electric field based devices for use in vaccine delivery, vaccine production, personal vaccine production, gene therapy, and hybridoma production. 
In recent years, there have been great advances in immunotherapy and gene therapy against such difficult targets as metastatic cancer, genetic defects, and acute viral infections. In many cases these advances have relied on the use of DNA and RNA. Today there are still no approved clinical uses of this technology because of the inability to deliver, safely and effectively, the DNA/RNA into the target cells. Cyto Pulse, a medical device company, has solved this problem with proprietary technologies using non-viral electric fields. After extensive preclinical studies these are now entering several human trials. The principal Cyto Pulse applications are the production of DNA therapeutic vaccines, delivery of DNA vaccines, modification of cell genomes to correct genetic defects, production of hybridoma cell vaccines by fusing immune system cells and tumor cells, and the production of hybridomas used to produce monoclonal antibodies. The annual markets for these treatments and vaccines are expected to be in the billions of dollars per year. The Cyto Pulse expertise and intellectual property is in waveform generators and electrodes for use in medicine and bioprocessing.
Management
Cyto Pulse Sciences, Inc. was founded by Richard Walters, MSEE, MBA and Alan King, DVM, Ph.D. Mr. Walters worked for the Department of Defense for 13 years, then founded a signal processing company which was eventually acquired by a NASDAQ company. Dr. King retired as a Lt Col. in the Army from the Infectious Diseases Branch at WRAIR. His expertise is in immunology and infectious diseases. Over the years he has spent time conducting infectious disease research in the Thailand, Peru, and Haiti.
Richard E. Walters, MSEE, MBA President and CEO 
Alan D. King, DVM, PhD Vice President 
John B. Burke, CPA, MBA Secretary and CFO 
Derin C. Walters, BSE, MBA (in process) Vice President 
Anna-Karin Roos, PhD Manager EU Operations
Facility
Cyto Pulse has a new 7000 square foot facility with a large bio laboratory, R&D electronics laboratory, QSR production facility and 200 square foot ISO 14644 compliant 'Class 5' clean room with sterilization apparatus. Cyto Pulse is located in the Cromwell Industrial Park adjacent to BWI Airport.